Physiol Rep. 2026 Oct;14(20):e71138. doi: 10.14814/phy2.71138.
ABSTRACT
Arterial stiffness and endothelial dysfunction are non-traditional risk markers after stroke, yet reliability and minimal detectable change (MDC) of pulse wave velocity (PWV) and flow-mediated dilation (FMD) have not been established. We examined test-retest reliability and MDC of carotid-femoral (cfPWV), carotid-radial (crPWV), and femoral-dorsalis pedis PWV (ffPWV), and absolute and relative brachial artery FMD in people >12 months post-stroke. Seventeen individuals (n = 4 (24%) females; 63.9 ± 8.6 years; 4.5 ± 2.8 years post-stroke) completed two bilateral assessments of PWV via applanation tonometry and FMD via duplex ultrasound within 1 week. Intraclass correlation coefficients (ICC) were calculated via two-way random-effects models, and MDC derived at 68%, 90%, and 95% confidence levels. We found good-to-excellent reliability for cfPWV in paretic (ICC = 0.85; 95% CI:0.59, 0.95) and non-paretic (ICC = 0.94; 95% CI:0.82, 0.98) limbs, with MDC values of 2.23 and 1.68 m/s, respectively. crPWV and ffPWV exhibited poor reliability bilaterally (ICC = 0.12-0.33). Relative FMD showed moderate reliability for paretic (ICC = 0.50; 95% CI: -0.20, 0.85) and non-paretic (ICC = 0.59; 95% CI:0.04, 0.84) limbs, with MDC values of 6.88% and 7.65%, respectively. Reliability for absolute FMD was poor (ICC = 0.11-0.44). These findings support cfPWV as a reliable vascular assessment in individuals >12 months post-stroke, and provide stroke-specific MDC values to guide interpretability in future trials.
PMID:42897953 | DOI:10.14814/phy2.71138