Wiad Lek. 2025;78(10):2034-2044. doi: 10.36740/WLek/210072.
ABSTRACT
OBJECTIVE: Aim: This study investigates the protective effects of Carbenoxolone (Cbx) in attenuating renal injury through the modulation of the FOXO3 gene.
PATIENTS AND METHODS: Materials and methods: Twenty-eight adult male Wistar Albino rats were randomly divided into four groups (N=7): Sham (laparotomy without ischemia), Control (bilateral renal ischemia for 30 min followed by 2 h reperfusion), Vehicle (DMSO injection prior to IRI), and Carbenoxolone-treated (Cbx injection three days before IRI).
RESULTS: Results: Tissue levels of TNF-α, IL-1β, MDA, Caspase-3, and KIM-1 were significantly elevated in the control and vehicle groups, while GSH levels were reduced. Cbx treatment significantly decreased TNF-α, IL-1β, MDA, Caspase-3, and KIM-1 while increasing GSH levels, indicating enhanced antioxidant defense. FOXO3 expression was notably lower in the Cbx group compared to the control and vehicle groups. Histopathological analysis further confirmed reduced renal damage in Cbx-treated rats. These findings suggest that Cbx exerts renoprotective effects by mitigating oxidative stress, inflammation, and apoptosis.
CONCLUSION: Conclusions: Carbenoxolone significantly reduces RIRI-induced kidney damage, demonstrating its potential as a therapeutic agent for renal ischemic injuries.
PMID:41401321 | DOI:10.36740/WLek/210072