Ir J Med Sci. 2026 May 18. doi: 10.1007/s11845-026-04443-7. Online ahead of print.
ABSTRACT
BACKGROUND: Premature coronary artery disease (PCAD) is marked by accelerated progression of atherosclerosis, an increased incidence of adverse cardiovascular events, and a less favorable long-term prognosis.
AIMS: The diagnostic potential and mechanistic basis of serum miR-20b-5p in PCAD.
METHODS: Serum levels of miR-20b-5p, sd-LDL indicators (including particle number, concentration, proportion), and LDL particle diameter were measured. Logistic regression identified risk factors for coronary stenosis severity. Spearman analysis assessed the association between miR-20b-5p and Gensini score. ROC curve analysis evaluated clinical diagnostic utility of miR-20b-5p. Cell experiments validated the regulatory interaction between miR-20b-5p and CCL1, and their effects on oxidative stress and inflammation.
RESULTS: The sd-LDL index and miR-20b-5p levels were significantly altered in patients with PCAD. Furthermore, miR-20b-5p levels and sd-LDL particle count were also associated with the severity of coronary stenosis.Serum levels of miR-20b-5p showed a significant negative correlation with the Gensini score and could serve as a diagnostic biomarker. miR-20b-5p directly targeted and inhibited CCL1, and its overexpression attenuated ox-LDL-induced oxidative stress and inflammatory reactions in HCAECs.
CONCLUSIONS: miR-20b-5p and abnormal small dense low-density lipoprotein are risk factors for evaluating the severity of coronary artery stenosis. Moreover, miR-20b-5p may alleviate vascular endothelial oxidative stress and inflammation by targeting CCL1, making it a potential therapeutic target for early intervention.
PMID:42144500 | DOI:10.1007/s11845-026-04443-7