Npj Imaging. 2026 Apr 7;4(1):23. doi: 10.1038/s44303-026-00156-9.
ABSTRACT
Extracellular arterial activity of the pro-inflammatory enzyme myeloperoxidase (MPO) destabilizes atherosclerotic plaque and associates with future atherothrombosis. To facilitate first-in-human studies using extracellular MPO activity as a molecular imaging target to identify high-risk atherosclerotic plaque, we describe [68Ga]Ga-IEMA, a NODAGA-based positron emission tomography (PET) radiotracer that provides an index for extracellular MPO activity. Synthesis of [68Ga]Ga-IEMA was achieved in five steps and with high radiolabelling efficiency. [68Ga]Ga-IEMA self-oligomerized and bound to proteins upon exposure to enzymatically active MPO, did not cross-cell membranes and was stable in human serum in vitro, while [68Ga]Ga-IEMA had favorable blood kinetics and stability in circulation in vivo. [68Ga]Ga-IEMA PET imaging in a mouse model of plaque instability revealed enhanced signal in unstable compared with stable plaque and plaque-free arteries. These data indicate that [68Ga]Ga-IEMA is a promising translational candidate for the non-invasive identification of high-risk atherosclerotic plaques and the evaluation of therapies targeting arterial inflammation.
PMID:41946800 | DOI:10.1038/s44303-026-00156-9