Wiad Lek. 2025;78(10):2101-2109. doi: 10.36740/WLek/210071.
ABSTRACT
OBJECTIVE: Aim: To evaluate nephroprotective effects of coenzyme Q10 in rats with renal ischemia -reperfusion injury by action on Notch-1 pathway.
PATIENTS AND METHODS: Materials and Methods: Twenty eight Wistar albino male rats were divided randomly into four groups (each group include seven rats): first one named sham group ( just laparotomy, no induction of ischemia), second one named control group (30 min. ischemia/ 2 hours reperfusion), third one named olive oil group (rats injected intraperitoneally. with 0.1 ml olive oil once daily for 2 days, 1 hour after the administration of olive oil in 3rd day renal ischemia was induced), fourth one named Coenzyme Q10 group (rats injected with Coenzyme Q10 (10 mg/kg) intraperitoneally. once daily for 2 days, 1 hour after the administration of Coenzyme Q10 in 3rd day renal ischemia was induced). Kidneys were harvested after 2 hours of reperfusion for assessment of kidney injury molecule-1, interleukin 1 β, TNF α, F2 Isoprostane, glutathione, and caspase 3 by ELISA technique, Notch-1, Jagged-1 mRNA level were assessed by reverse transcription-polymerase chain reaction.
RESULTS: Results: Coenzyme Q10 significantly ameliorated renal ischemia -reperfusion injury as evidenced by the significant reduction of kidney injury molecule-1, interleukin 1 β, TNF α, F2 Isoprostane, and caspase 3 in rat kidneys pretreated with Coenzyme Q10, glutathione level was significantly elevated in rat kidneys pretreated with Coenzyme Q10. Notch-1 and Jagged-1 mRNA expression were significantly decreased compared to control group.
CONCLUSION: Conclusions: Coenzyme Q10 provide nephroprotection against renal ischemia reperfusion injury owing to its antioxidant, anti-inflammatory, and anti-apoptotic properties, those effects may be linked to the downregulation of Notch-1 and Jagged-1.
PMID:41401329 | DOI:10.36740/WLek/210071